Hemoglobin is the iron-containing protein, which carries oxygen in your bloodstream. RetinaLyze Glaucoma measures the relative hemoglobin amount in the optic disc in relation to the main retinal vessels.
The greater the thickness of the tissue or its concentration of hemoglobin, the more intense the color, and the thinner the tissue or the less abundance of blood, the whiter it will appear. Thus, the concentration of hemoglobin in the optic disc can be used as an indication of the presence of glaucoma.
Below is an image, showing the difference in hemoglobin (relative to the the vessels) between a normal optic disc and glaucomatous optic disc. 100% on the scale refers to the hemoglobin level in the central vessels of the retina. Warmer colors represent high densities of hemoglobin and cooler colors represent areas of lower perfusion or thin tissue.
RetinaLyze Glaucoma assesses the level of hemoglobin in the ONH (arteries, veins, rim and cup) by assessing the colours in the fundus image. The vessels are used as reference for calibration.
Finally, a Glaucoma Discriminant Function (GDF) is calculated to assess if there is damage to the ONH, which indicates the presence of glaucoma.
For a more in depth explanation of the way the RetinaLyze Glaucoma algorithm works, check out the article below: